Duopath® Cereus Enterotoxins from Merck
The world’s most convenient manual test for rapid screening and confirmation of Bacillus Cereus
With Duopath® Cereus Enterotoxins, Merck offers the only lateral flow test worldwide for the convenient detection of Bacillus cereus via the enterotoxins HBL and NHE.
In recent years Bacillus cereus has become more and more relevant as a foodborne pathogen causing gastrointestinal diseases. Susceptible food products are meat and milk products, vegetables, soups, spices and especially baby food. Important for the food industry are especially those strains of B. cereus that are able to produce particular enterotoxins causing a diarrhoea-associated food intoxication. These intoxications are mainly caused by the major enterotoxins haemolysin BL (HBL) and the non-hemolytic enterotoxin (NHE) produced by B. cereus during their intestinal passage in the human gut. NHE is produced by more than 90 percent and HBL by about 55 percent of B. cereus isolates. Rapid test systems commercially available so far for detection of Bacillus cereus rely on the production of either HBL or NHE only.
The convenient solution from Merck
Duopath® Cereus Enterotoxins is a reliable, rapid immunological screening and confirmation test for the simultaneous detection of both the most important enterotoxins of B. cereus, HBL and NHE with one single test strip.
Based on the immune flow principle, Duopath® Cereus Enterotoxins is designed to avoid time-consuming and manpower-intensive working steps. No instrument is required to run the test or to interpret the test result.
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Duopath® Cereus Enterotoxins can be used for both rapid and sensitive screening of foods (detection limit 100 and 1 CFU/gram food or environmental sample, respectively) as well as quick confirmation of suspect B. cereus from selective agars within 4.5 hours (detection limit 1 colony on agar plate). Using Duopath® Cereus Enterotoxins from Merck, you benefit from a number of major advantages compared to other methods. |
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| Reliable: | Due to the incorporation of monoclonal antibodies in the test format, Duopath® Cereus Enterotoxins from Merck is more specific than the existing ELISA or latex methods which employ polyclonal antibodies. Due to the simultaneous testing for two major enterotoxins of B. cereus with one assay, Duopath® Cereus achieves more accurate results. |
| Fast: | Duopath® Cereus Enterotoxins is the fastest manual confirmation test for enterotoxigenic Bacillus cereus, providing same-day results (within 4.5 hours). |
| Easy and convenient: | Simply add sample and read the result after only 30 minutes. The highly convenient handling of Duopath® Cereus Enterotoxins avoids working errors and significantly reduces the workload for your lab personnel. |
| Safe: | Our test delivers clear and distinct positive or negative results with a positive control reaction already built-in. |
| Economical: | Using our test, you do not need to invest in expensive instruments such as automated systems. Simple handling and rapid results save time and labour costs – even unskilled personnel can easily use Duopath® Cereus Enterotoxins. |
Comprehensive rapid test portfolio
Duopath® Cereus Enterotoxins extends Merck’s broad portfolio of manual rapid tests for the most common food and environmental pathogens. Our reliable, easy-to-use Singlepath® and Duopath® rapid test programme comprises lateral flow tests for the detection of E. coli 0157, verotoxin-producing E. coli, Salmonella, Campylobacter, Listeria, Listeria monocytogenes, Legionella, and B. cereus. What is more, we offer dehydrated and ready-to-use culture media especially adapted to our tests for precise and reliable results.
For use with Duopath® Cereus Enterotoxins, we have designed a new high-quality CGY enrichment broth: Using this broth supplemented with 1% glucose (final concentration in broth) for enrichment, toxin production in the sample is drastically enhanced, thus rapid and reliable results with Duopath® Cereus Enterotoxins are guaranteed.
If you are looking for an extremely easy-to-use and highly reliable rapid test for enterotoxigenic Bacillus cereus, look no further: Duopath® Cereus Enterotoxins from Merck is your most convenient and economical solution.
For more information, please contact us.
