Choose ZIC^®-HILIC HPLC columns to separate your polar and hydrophilic compounds

Merck Millipore’s SeQuant™ ZIC^®-HILIC HPLC columns provide the solution you’ve been seeking to separate your polar and hydrophilic compounds. Here you can combine the benefits of using aqueous-based eluents with separation according to normal phase principles. And all of this on a reliable high-performance stationary phase.

Separating charged and neutral hydrophilic compounds, such as acids, bases, ions, peptides, metabolites and sugars, can be very difficult using reversed phase HPLC. But thanks to its hydrophilic zwitterionic separation selectivity, ZIC^®-HILIC makes separating these compounds much easier. The robust patented bonding chemistry makes ZIC^®-HILIC a very versatile tool for separating all types polar and hydrophilic compounds. Select your ZIC^®-HILIC HPLC column from a comprehensive range of dimensions - from capillary through analytical to semi-preparative.

HILIC stands for hydrophilic interaction liquid chromatography, a HPLC separation technique that brings benefits in your analysis of polar and hydrophilic compounds, as it is easy to combine with a wide range of detection techniques. What’s more, HILIC boasts improved sensitivity with some of these techniques, such as electrospray ionization mass spectrometry (ESI-MS). Visit the HILIC Technology Tutorial to learn more about the possibilities with the technique and the advantages this gives you in separating polar and hydrophilic substances.

For the extra demanding applications, the polymer-based ZIC^®-pHILIC HPLC columns is an alternative choice when you require a wider range of pH conditions to fine-tune the separation selectivity for your polar and hydrophilic compounds.

ZIC^®-HILIC has been successfully used to separate compounds such as acids and bases, anions and cations, carbohydrates, metabolites, metal complexes, amino acids, peptides, protein digests and many more. Some recent applications using ZIC^®-HILIC can be found in the documents listed below:

• ZIC^®- HILIC Separation of Sucrose with RI detection
• ZIC^®- HILIC Separation of Fructose and Sucrose
• ZIC^®- HILIC Separation of tryptic peptides from Ovalbumin

A comprehensive collection of ZIC^®-HILIC application notes can be found on the SeQuant Applications page.

Detailed instructions for using ZIC^®-HILIC columns can be found in the documents listed below. More support documents can be found in the SeQuant™ download area.

• ZIC^®- HILIC Analytical HPLC Columns
• ZIC^®- HILIC Nano, Capillary and Microbore HPLC Columns
• ZIC^®- HILIC Semi-Preparative HPLC Columns

For technical support on ZIC^®-HILIC products or questions regarding application development, please consult the ZIC^®-HILIC FAQ list or contact the SeQuant™ Support Team.